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IACUC Routes of Administration Guidelines

MOUSE

IV bolus: 1-5 ml/kg; less than 0.2 ml total
IV drip: 2-4 ml/kg/hr
IP: 2-3 ml
SC: 2-3 ml in the scruff, 0.2 ml inguinal
ID: <0.05 ml/site
IM: ≤50 microliters/site
IN: <0.02 ml
Gavage: 10 ml/kg
ED: 0.15-0.2 ml/kg
RO: 200 ul

If mice are under 21 days of age, contact RAR for recommended injection volumes
 

RAT

PO: 5 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 10-20 ml/kg
SC: 5-10 ml/kg or 5-10 ml total over 2-4 sites
ID: 0.05-0.1 ml/injection
IM*: 0.1-0.2 ml maximum
IN: 50 ul
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg
 

GUINEA PIG

PO: 5 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.03-0.05 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

HAMSTER

PO: 5 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.03-0.05 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

FERRETS

PO: 100 ml/session (10 ml/min)
IV bolus: 2-5 ml/kg, 10 ml/kg via slow injection
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/site
IM: 0.05 ml/kg/site
IN: 0.03-0.05 ml/injection
Gavage: (1-20 ml/kg), maximum of 50 ml total
ED: 0.15-0.2 ml/kg
 

RABBIT

PO: 5 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-10 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.

DOG

PO: 5-20 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.

CAT

PO: 5-20 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

MACAQUE

PO: 5-20 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

SWINE

PO: 5-20 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05-0.1 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

SHEEP/RUMINANTS

PO: 5-20 ml/kg
IV bolus: 1-5 ml/kg
IV drip: 2-4 ml/kg/hr
IP: 1-10 ml/kg
SC: 1-5 ml/kg
ID: 0.05-0.1 ml/injection
IM*: 0.05-0.1 ml/kg/site
IN: 0.2-0.5 ml/injection
Gavage: 1-20 ml/kg
ED**: 0.15-0.2 ml/kg

*Maximum single IM dose per site is 5 ml for animals >10 kg. If the volume exceeds 5 ml, give over multiple sites.
**For epidural, no more than 6 ml total volume for animals up to 35 kg.
 

MOUSE

IV: Lateral tail 25G
IM: Not recommended
SQ: 20G
IP: 25G
 

RAT

IV: Lateral tail 23G
IM: Not recommended
SQ: 20G
IP: 21G
 

GUINEA PIG

IV: Ear vein, saphenous - 23G
IM: Quadriceps - 21G
SQ: 20G
IP: 21G
 

HAMSTER

IV: 25G (no peripheral vessel recommended)
IM: Not recommended
SQ: 20G
IP: 21G
 

FERRET

IV: Cephalic, jugular vein - ≤23G, 5/8-1’ long
IM: Quadriceps, epaxial - ≤21G, 5/8” long
SQ: ≤21G
IP: ≤21G, 1.5” long
 

RABBIT

IV: Marginal ear - 21G
IM: Quadriceps, epaxial - 20G
SQ: 20G
IP: 20G
 

DOG

IV: Cephalic - 21G
IM: Quadriceps, epaxial - 20G
SQ: 20G
IP: 20G
 

CAT

IV: Cephalic - 21G
IM: Quadriceps, epaxial - 20G
SQ: 20G
IP: 20G
 

MACAQUE

IV: Cephalic, saphenous - 20G
IM: Quadriceps/posterior thigh - 20G
SQ: 20G
IP: 20G
 

SWINE

IV: Marginal ear vein - 20G
IM: Neck/epaxial, gluteal muscles - 20G
SQ: 20G
IP: 20G
 

SHEEP/RUMINANTS

IV: Jugular, saphenous - 20G
IM: Quadriceps, epaxial, gluteal muscles - 20G
SQ: 20G
IP: 20G
 

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